Isingeniso

Ezilabhorethri zanamuhla, ama-autosampler vials abe ithuluzi elibalulekile ekuqinisekiseni ukuthi ukuhlola kusebenza kahle, kunembile futhi kuthembekile.Kungakhathaliseki ukuthi ekuhlaziyeni amakhemikhali, ukuqapha indawo ezungezile noma ucwaningo lomtholampilo, izitsha ze-autosampler zidlala indima ebalulekile, zisebenza ngokubambisana namabhodlela e-autosampler ukuze kuqoqwe futhi kugcinwe amasampula ngokushesha nangokunembile. Lokhu kusebenza okuzenzakalelayo akuthuthukisi kakhulu ukusebenza kahle kokuhlolwa futhi kunciphisa amaphutha abantu, kodwa futhi kuqinisekisa ukuvumelana kwesampula kanye nekhwalithi.

Kodwa-ke, naphezu kokulula okulethwa yizitsha ze-autosampler, kusenezinkinga ezivamile ezingase zenzeke ngesikhathi sokusetshenziswa kwazo. Lezi zinkinga zingase zithinte ubuqotho besampula noma ukunemba kwemiphumela yokuhlola, ngaleyo ndlela kuthinte ukwethembeka kwayo yonke inqubo yokuhlaziya.

Ngakho-ke, inhloso yalesi sihloko iwukuxoxa ngezinkinga ezivamile ezingase zihlangabezane nazo lapho kusetshenziswa izitsha ze-autosampler kanye nokuhlinzeka amalabhorethri ngochungechunge lwezixazululo ezisebenzayo zokuqinisekisa inqubo yokuhlola ebushelelezi kanye nokukhulisa ukunemba kokuhlaziya nokuthembeka kwemiphumela.

Uhlolojikelele lwemibuzo evamise ukubuzwa mayelana ne-Autosampler Vials

1. Ikepisi lamabhodlela liyavuza noma alivali kahle

Ukusebenza kokuvalwa kwekepisi kuthinta ngqo ukusebenza kwe-autosampler vials. Uma ikepisi lingavalwanga kahle noma uphawu lunesici, isampula ingase ivuze noma ihwamuke, okuholela ekulahlekeni kwesampula, ukuguquguquka kwezingxenye noma ngisho nokungcoliswa kwangaphandle. Ukuvalwa kahle kungaholela emoyeni noma ezinye izinto zangaphandle ezingena ebhodleleni, okuthinta ikhwalithi yesampula.

2. Izitsha ze-autosampler eziphukile noma ezilimele

Izitsha ze-autosampler zivame ukwakhiwa ngengilazi, okuthi, naphezu kokuzinza kwayo okuhle kwamakhemikhali nokungafihli, kujwayele ukuphuka ngesikhathi sokuthutha, ukuphatha noma ukuhlanza. Noma imuphi umthelela wangaphandle, ukushintsha kwezinga lokushisa, noma umehluko wengcindezi kungase kubangele ukuba ibhodlela noma umlomo uphuke, futhi ibhodlela lesampula eliphukile lingaholela ekuvuzeni kwesampula noma umonakalo, okuholela ekulahlekeni kwedatha yokuhlola. Ngesikhathi esifanayo, izingcezu zezingilazi eziphukile zingase zibe yingozi yokuphepha kubasebenzi baselabhorethri, zandise ukuguga nokudabuka kwemishini kanye nokuba yinkimbinkimbi kokusebenza.

3. Ukungcola okuyisampula

Ukukhetha okungalungile kwempahla ye-autosampler vials noma amakepisi angcolile kungaholela ekungcolisweni kwesampula. Amakhemikhali athile angase asabele ngento yamabhodlela noma akhangiswe ngodonga lwamabhodlela, okuthinta ukuhlanzeka kwesampula. Ukwengeza, izindlela zokuhlanza ezingalungile noma izindawo zokugcina zingaholela ekukhuleni kwezinsalela noma amagciwane ngaphakathi kwebhodlela, okungangcolisa isampula. Amasampuli angcolile angathinta ngokuqondile ukuthembeka kokuhlolwa, okuholela kudatha ehlanekezelwe futhi kuthinte ukunemba kwemiphumela yokuhlaziya.

4. Izimo ezingalungile zokugcina izitsha ze-autosampler

Izimo zokugcina izitsha ze-autosampler zibalulekile ekusebenzeni kwazo kanye nekhwalithi yesampula. Izimo ezingafanele zokugcina (isb., izinga lokushisa eliphezulu, umswakama ophezulu, ukukhanya kwelanga eliqondile, noma izindawo ezimanzi ngokweqile) kungase kubangele ukonakala kwempahla yebhodlela noma kuthinte ukuqina kwamasampula ngaphakathi kwebhodlela, futhi amanye amasampula azwela amakhemikhali angase asabele noma abole ngenxa yezimo ezingafanele zokugcinwa; kuyilapho izimo ezingezinhle zokugcina zingaholela ekushintshashintsheni kwebhodlela, ukwehluleka kwezigxivizo, noma ngisho nokugqabuka. Amasampuli angase awonakale noma angcoliswe ezindaweni ezingafanele, ekugcineni athinte ukufaneleka kokuhlolwa kanye nokunemba kwedatha.

Lezi yizinhlobo ezinhlanu ezivame kakhulu zezinkinga ezingase zibe nomthelela ekusebenzeni kwezitsha ze-autosampler futhi ngokwezinga elithile kufanele zithinte ukunemba kwemiphumela yokuhlola.

Izixazululo Nezincomo

1. Isixazululo 1: Qinisekisa ukuvala uphawu

Hlola njalo ama-cap seal ukuze uqiniseke ukuthi awagqoki noma awonakele, ikakhulukazi uma usebenzisa imvamisa ephezulu. Khetha amakepisi wekhwalithi engcono ukuze uqinisekise ukusebenza okuzinzile kokuvala, futhi uqinisekise indlela efanele yokudonsa, futhi ugweme amandla amaningi uma ufaka amakepisi, angase athinte umumo noma umsebenzi wophawu.

Ama-caps anezimpawu ezikhethekile angasetshenziswa, anikeze uphawu olungcono futhi anciphise amathuba okuvuza kwegesi noma ukuhwamuka kwesampula. Okunye ukuhlola okunembe kakhulu kungase kudinge ukusetshenziswa kwezindlela zokuvala ezengeziwe ukuze kuqinisekiswe ukuqukatha okuphelele kwesampula, ikakhulukazi ezintweni eziguquguqukayo.

2. Isixazululo 2: Khetha impahla yebhodlela efanele kanye nokucaciswa

Khetha okokusebenza okufanelekile kwe-autosampler vials ngokuya ngohlobo lwesampula elisetshenziswe ekuhlolweni. Ukukhetha into ezinzile ngamakhemikhali kubaluleke kakhulu uma isampula isebenza ngamakhemikhali kakhulu noma iguquguquka. Amabhodlela engilazi afaneleka kakhulu ekuhlaziyweni okujwayelekile, kodwa kumasampuli angaphansi kwezimo ezithile ezimbi kakhulu (isb., izixazululo ezine-asidi, ezine-alkaline noma izindawo ezishisa kakhulu, njll.), amabhodlela e-polypropylene noma ezinye izinto ezikhethekile ezicutshunguliwe zingase zifaneleke kakhulu.

Hlola ngezikhathi ezithile ukubukeka kwamabhodlela ukuze uqiniseke ukuthi akukho mifantu noma omunye umonakalo, ikakhulukazi ngesikhathi sokuthutha nokugcinwa. Amabhodlela engilazi asengozini yokuphuka ngenxa yamandla angaphandle, futhi ukuhlola okuvamile kungasiza ekuvimbeleni ukuvuza noma ukulahlekelwa amasampula ngenxa yokulimala kwamabhodlela. Gwema ukushisa noma imithelela ngesikhathi sokugcinwa, okunganweba impilo yebhodlela.

3. Isixazululo 3: Ukuhlanza nokunakekela

Ukuhlanzwa kwama-autosampler vials kuyisinyathelo esibalulekile sokuqinisekisa ukuthi amasampula awangcoli. Hlanza kahle amabhodlela njalo, ikakhulukazi ngemva koshintsho lwesampula noma ngemva kokuba amabhodlela egcinwe isikhathi eside. Gwema ukungcolisa amasampula amasha ngamakhemikhali ayinsalela, izinyibilikisi noma izinto zokuhlanza.

Lapho uhlanza, hlambulula ibhodlela kahle nge-solvent efanele. Ngemuva kokuhlanza, qiniseka ukuthi izitsha zengilazi zebhodlela zomile ngokuphelele, ngokusebenzisa indwangu ehlanzekile engalukiwe noma okomisa umoya. Kubalulekile futhi ukuhlanza amakepisi nezintamo zamabhodlela ngesikhathi sokuhlanza ukuvimbela izinto ezingcolile ukuthi zingathinti ikhwalithi yamasampula.

4. Isixazululo 4: Naka izimo zokugcina

Izitsha ze-autosampler kufanele zigcinwe endaweni efanelekile, kugwenywe izimo ezingezinhle ezifana nokushisa, umswakama noma ilanga eliqondile. Indawo ekahle yokugcina indawo enezinga lokushisa elimaphakathi kanye nomswakama ophansi, ovimbela ukuwohloka kwempahla yebhodlela noma ukonakala kwekhwalithi yesampula.

Ukuze ugweme ukushintshashintsha kwezinga lokushisa kanye nemiphumela yomswakama, cabanga ukusebenzisa amabhokisi okulondoloza akhethekile noma amaphakheji okuvikela. Lawa maphakheji avikela ngokuphumelelayo amabhodlela ekushintsheni kwendawo yangaphandle futhi aqinisekise ukuthi ikhwalithi igcina ukusebenza kwayo nokuvala ngesikhathi sokugcina. Kumasampula adinga ukugcinwa isikhathi eside, kunconywa ukusebenzisa amakhabethe okugcina ama-cryogenic noma imishini yokugcina ehambisana nokucindezela komoya.

Izixazululo ezingenhla zingagwema ngokuphumelelayo izinkinga ezingase zenzeke ekusetshenzisweni kwe-autosampler vials futhi zithuthukise ukuthembeka kokuhlolwa kanye nokunemba kokuhlaziywa kwesampula. Ukuqinisekisa ukuvalwa kwekepisi, ukukhetha impahla yebhodlela efanele kanye nokucaciswa kwayo, ukuhlanza nokugcinwa njalo, nokuqinisekisa ukuhambisana kwekhwalithi nezinto zokusebenza kuyizici ezibalulekile ekuqinisekiseni ukuthi ukuhlola kusebenza kahle.

Amanothi Engeziwe Namathiphu

1. Ukuhlolwa njalo nokulinganiswa kwemishini

Hlola ngezikhathi ezithile izingxenye ezihlanganisiwe ze-autosampler nebhodlela ukuze uqinisekise ukuthi zonke izixhumanisi noxhumana nabo akugqoki noma konakaliswe. Ama-autosamplers ngokuvamile aqukethe izingxenye eziningi ezihambayo ezingase ziguge uma zisetshenziswa, okuholela ekutheni amabhodlela angahlangani kahle noma angavali kahle. Ngakho-ke, ukuhlolwa okuvamile nokulinganisa kuyisinyathelo esidingekayo ukuze kuqinisekiswe ukunemba kwemishini.

Ngaphezu kokulinganisa okokusebenza kwemishini, ukunemba kwesampula kudinga ukulinganiswa ngezikhathi ezithile ukuze kuqinisekiswe ukuvumelana nokwethembeka ekusebenzeni kwayo. Ukulinganisa okuvamile akuthuthukisi nje kuphela ukunemba kwesampula, kodwa futhi kwandisa impilo yesisetshenziswa.

2. Ukulandelela izincomo zomthengisi

Qonda futhi ulandele imiyalelo yokusetshenziswa enikezwe umenzi noma okokusebenza kwe-autosampler sula ukusabalala kokudabukisayo okuhle ukudabukisayo. Le mihlahlandlela ivamise ukuqukatha ulwazi olumayelana nendlela engcono kakhulu yokusebenzisa okokusebenza, izikhawu zokulungisa, kanye nezinkinga ezijwayelekile nezisombululo okungase kuhlangatshezwane nazo ngesikhathi sokusetshenziswa. Izincomo zabakhi ziyindlela engcono kakhulu esekelwe ocwaningweni lwesikhathi eside nokuhlola, ngakho ukulandela izincomo ezinikezwe umenzi eduze kuzoqinisekisa ukusebenza kahle kwemishini namabhodlela.

Izinhlobo ezihlukene zamabhodlela engilazi kanye nokuthathwa kopende we-matte othomathikhi kungase kube nomehluko ekwakhiweni kwemininingwane, ngokuya ngezidingo eziqondile zomkhiqizi okufanele zisetshenziswe, hhayi nje ukugwema ukungasebenzi kahle okulethwa ukusebenza okungalungile, kodwa futhi nokuqinisekisa ukunemba kwedatha kunqubo yokuhlola.

3. Ukuphatha kahle iqoqo

Kumalabhorethri asebenzisa inani elikhulu lama-autosampler vials, ukuphathwa kahle kwenqwaba kubalulekile. Amaqoqo ahlukene amabhodlela angase abe nomehluko omncane wezinto ezibonakalayo, usayizi noma inqubo yokukhiqiza, ngakho-ke kubalulekile ukuhlukanisa ngokuqinile phakathi kwamaqoqo lapho uwasebenzisa ukuze ugweme ukudida umthombo kanye nokufaka engozini ukunemba kwamasampuli.

Lokhu kungenziwa ngohlelo lokuphatha amalebula noma ngokubhala ikhodi emaphakathi neqoqwana ukuze kuqinisekiswe ukuthi iqoqo ngalinye lamabhodlela engilazi lisetshenziswa ngendlela efanele ngokuya ngokucaciswa kwalo kanye nemibandela yokusetshenziswa kwalo. Kufanele futhi kuqashelwe ukurekhoda isikhathi sokusetshenziswa nesimo samabhodlela ukuze umlando nokusetshenziswa kwamabhodlela kulandelelwe lapho kudingeka.

4. Ezinye izinto kanye nobuchwepheshe obusha

Njengoba ubuchwepheshe buthuthuka, izinto ezintsha ze-autosampler vials ziyaqhubeka nokuphuma, eziningi zezinto ezintsha zodonga lwengilazi yendabuko kanye nepulasitiki kunenzuzo enkulu. Isibonelo, izinga lokushisa eliphezulu nezinto eziyinhlanganisela ezimelana namakhemikhali zingamelana nezimo zokuhlola eziqinile, ezifanele uhla olubanzi lwezimo zohlelo lokusebenza. Kwezinye izinhlelo zokusebenza ezikhethekile, ungacabangela izinto ezintsha zokusebenzisa izitsha zesampula ukuze uthuthukise ukunemba nokuphepha kokuhlolwa.

Izinto ezithile eziyinhlanganisela, ezinokumelana nokushisa okuphezulu nokugqwala, zingahlala zizinzile emazingeni okushisa aphezulu noma ezindaweni ezine-asidi eqinile ne-alkali. Ukwengeza, amanye amapulasitiki asebenza kahle kakhulu awagcini nje ngokuba nokumelana kwamakhemikhali okuhle kakhulu, kodwa futhi anciphisa ngokuphumelelayo ukusabela phakathi kwesampula nodonga lwamabhodlela, alungele ukusebenza kwesampula ezindaweni ezimbi kakhulu.

Ngalezi zinyathelo zokuphepha ezengeziwe namathiphu, amalebhu angathuthukisa ukusebenza kahle kwama-autosampler vials awo, awasize akhulise ukusebenza kahle kwelebhu, andise impilo yemishini, anciphise amaphutha, futhi aqinisekise izinga eliphezulu lokunemba kudatha yawo yelebhu.

Isiphetho

Izitsha ze-autosampler zidlala indima ebalulekile kumalabhorethri esimanje, futhi ukusetshenziswa kwazo okufanele nokunakekelwa kuhlobene ngokuqondile nokunemba nokuthembeka kwemiphumela yokuhlola. Ngokukhetha okufanele, ukuhlola nokugcinwa okuvamile, ukubekwa uphawu, ukuqina kanye nokuvumelana nezimo kwezitsha ze-autosampler kungaqinisekiswa, futhi izinkinga ezivamile zingagwenywa, ngaleyo ndlela kuthuthukiswe ukusebenza kahle kokuhlolwa kanye nokwethembeka kwemiphumela.

Kuphela ngokukhethwa kwesayensi nokugcinwa ngokucophelela, izitsha ze-autosampler zinganikeza ukudlala okugcwele ezinzuzweni zazo eziphezulu, zisize amalabhorethri ukuthi aqedele imisebenzi yokuhlaziya ngayinye ngendlela efanele futhi enembile, ngaleyo ndlela anikeze ukusekelwa kwedatha okunamandla ocwaningweni lwesayensi nokukhiqizwa kwezimboni.