Isingeniso

Kulabhorethri yesimanje, ukusebenza ngokunemba kubeka izidingo ezikhulayo ezitsheni. Ikakhulukazi lapho besebenza ngokulandela amanani oketshezi, opharetha bavame ukubhekana nezinselele eziningi. I-labware evamile, nakuba isabalulekile ekusebenzeni okujwayelekile, inkulu futhi ayinembile lapho uphatha imithamo emincane yoketshezi, okwenza kube nzima ukuhlangabezana nezidingo eziqinile zokunemba nokuhlanzeka ezimweni zokuhlola.

Idizayini eneziqu eziphakeme kakhulu zebhodlela le-dropper elineziqu zevolumu encane yenza ukukhishwa kwe-liquid kulawuleke futhi kuthembeke.

Kungani i-Lab ingakwazi ukwenza ngaphandle kwamabhodlela ama-Burette Amancane Athweswe Iziqu?

Amabhodlela e-burette aneziqu ezincane asetshenziswa kakhulu ezindaweni zokucwaninga ngoba abonisa izinzuzo ezihlukile ngokunemba, ukuphepha nokusebenza kahle.

1. Ikhono lokukala ngokunemba

Ukuphindaphinda nokunemba kokuhlolwa kuncike ekungezeni uketshezi okunembile. Amabhodlela e-dropper akhethekile anenani lephutha elincane kunobubanzi kuneziqukathi ezithweswe iziqu ngokwesiko futhi angahlangabezana nezidingo zokuhlolwa ngokungezwa komkhondo okulawulwa ngokuqinile.

2. Umklamo wokulwa nokungcola

Ibhodlela le-dropper liklanywe nge-screw-seal cap noma ithiphu ye-dropper yesiqeshana esisodwa, ethuthukisa ngokuphawulekayo ukuvala futhi ivimbele ngempumelelo okuqukethwe ekuhwamukeni noma ekufakweni kwe-oxidizing. Ngesikhathi esifanayo, uma kuqhathaniswa nokusebenza kwe-pipette okudinga ukuguqulwa kwethiphu njalo, ithiphu ye-dropper ngokwayo igwema imisebenzi eminingi futhi inciphisa amathuba okungcola okuphambene, ukuthuthukisa ukusebenza kahle nokulondoloza izinto ezisetshenziswayo.

3. Ukuphepha kwezinto ezibonakalayo

Amabhodlela e-dropper aqediwe esiwathengisayo enziwe ngengilazi ephezulu ye-borosilicate, ukumelana nokushisa futhi ukumelana nokugqwala, alungele ukwelashwa okunokushisa okuphezulu noma ama-asidi aqinile nama-alkali.

Izimo Zokusebenza Ezijwayelekile

Amabhodlela e-burette anevolumu emincane asetshenziswa kabanzi emikhakheni eminingi yokuhlola ngokusebenziseka nokuvumelana nezimo, ikakhulukazi emisebenzini yokuhlola edinga ukunemba okuphezulu kokulawula uketshezi kanye nokusebenza kalula.

1. Ukuhlolwa kwebhayoloji yamangqamuzana

Emisebenzini yeleveli yamangqamuzana, ubukhulu nomthamo wama-reagents kuthinta ngokuqondile imiphumela yokuhlolwa. Amabhodlela e-Dropper alungele ukukhishwa nokugcinwa kwe-DNA/RNA, futhi usayizi we-1ml uvimbela ngempumelelo ukuhwamuka kwesampula futhi wenze kube lula ukugcinwa esiqandisini. Emisebenzini ye-enzyme noma ye-antibody, amabhodlela angu-3ml angasetshenziswa ukukhipha inani elifanele lama-reagents, ukugwema ukulahlekelwa umsebenzi okubangelwa ukuqhwa okuphindaphindiwe kanye nokuncibilika kwamabhodlela amakhulu, nokuqinisekisa ukuzala kabusha nokuzinza kokuhlolwa.

2. Ukuhlaziywa kwamakhemikhali

Ukulungiswa kwe-vial okujwayelekile ekuhlaziyweni komthamo, ibhodlela le-dropper elingu-5 ml linikeza isikhala sokubonwa kalula kanye nokukhohlisa futhi lifanele ukuhlanjululwa kwezigaba eziningi. Ngama-reagents athile anobuthi kakhulu noma aguquguqukayo, ithiphu lokuvuza lebhodlela elingavuzi kanye nomklamo wentambo yokuvala ithuthukisa ngokuphawulekayo ukuphepha kokuphatha futhi inciphisa ingcuphe yokuchayeka kwabasebenzi kanye nokuhwamuka kwegesi.

3. Amalabhorethri okufundisa

Emakolishi nasezifundweni zaselabhorethri yesikole samabanga aphakeme, ukukhishwa kwe-reagent kusengaphambili akukwazi nje ukunciphisa ngempumelelo imfucuza ye-reagent, kodwa futhi kunciphisa amathuba okuxhumana okuqondile kwabafundi namakhemikhali ayingozi futhi kuthuthukise izinga lemfundo yokuphepha. Amabhodlela abonisa ngale anezikali asiza abafundi ukuthi bathole ulwazi “lokubona ivolumu” kanye “nokunemba kwe-titration”, futhi bathuthukise ukuqeqeshwa kwamakhono okuhlola.

Umhlahlandlela Wokukhetha

Phakathi kwemikhiqizo eminingi nezinto ezisetshenziswayo ongakhetha kuzo, ukuthengwa kwesayensi nokunengqondo kwamabhodlela e-dropper anevolumu encane kubalulekile ukuze kuqinisekiswe ukwethembeka nokuphepha kwemiphumela yokuhlola.

1. I-logic yokukhetha umthamo

Izidingo zokuhlolwa kwe-opaque zikhomba osayizi bamabhodlela abashintshiwe:

• 1ml/2mlamabhodlela afaneleka kakhulu ama-reagents anenani elilodwa, ukunciphisa imfucuza kanye nokwenza lula ukugcinwa.
• 3mlamabhodlela awusayizi ovame kakhulu futhi wendawo yonke, afanele ukuhlolwa kwansuku zonke ekukhipheni uketshezi, umthamo omaphakathi futhi kulula ukuthwala.
• 5mlamabhodlela afanele izixazululo ezivamile, ukugwema ukugcwalisa ngokuphindaphindiwe kanye nokwenza ngcono ukusebenza kahle kokuhlolwa.

2. Ukugxila kwepharamitha engukhiye

Inqubo yokukhetha kufanele igxile kulokhu:

• Ukucaca kwesikali: Amabhodlela e-dropper ekhwalithi ephezulu kufanele aqoshwe nge-laser noma aphrintwe ngokunamathela okuphezulu ukuze kugwenywe ukufiphala kwesikali ekushayweni kwezinga lokushisa okuphezulu noma ukuhlanzwa kanye nokuqinisekisa ukufundeka kwesikhathi eside.
• Ukubeka uphawu: Kunconywa ukwenza isilingo esilula sokuguqula ngaphambi kokuthenga kokuqala - gcwalisa ibhodlela ngamanzi, uvale ikepisi ngokuqinile futhi uyiguqule amahora angu-24 ukuze ubone ukuthi kukhona yini ukuvuza, okusetshenziselwa ukulingisa isimo sangempela sokulondoloza.

3. Isexwayiso sokugwema izingibe

Izindawo zaselabhorethri zibeka izimfuno eziphezulu ezintweni zesitsha, futhi lezi zindaba ezilandelayo zidinga ukucaciswa:

• Amabhodlela epulasitiki asezingeni eliphansi angase abe nama-plasticizers noma i-organic solvent leachate, ikakhulukazi lapho egcina ama-acidic noma ama-organic reagents, angase athambekele ekungcoleni, okuthinta ubumsulwa nokuphepha kokuhlolwa.
• Imikhiqizo eshibhile enamaphutha esikali esikhulu ingase iholele kumthamo we-spiking ongalungile, okungase kubangele ukwenzelela esivivinyweni noma ukwehluleka ukuphindaphinda, ikakhulukazi uma uklama ukusabela okuzwela ukugxila.

Isiphetho

Amabhodlela amancane aneziqu ze-dropper awabonakali kodwa adlala indima ebalulekile endaweni eyinkimbinkimbi nesebenzayo yelabhorethri. Ngokulawula isikali esinembayo/ukusebenza okuhle kakhulu kokuvala kanye nezinto ezikhethwayo ezihambisana namakhemikhali, zinikeza isiqinisekiso esiphindwe kathathu “sokunemba+ukuphepha+kokusebenza” emisebenzini yokuhlola. Lawa magajethi ayisisekelo kodwa abalulekile aqinisekisa ukwethembeka kwedatha, ubuqotho bamasampuli, nokuphindaphindeka kwezinqubo zokuhlola.

Abahloli kufanele bakhethe umthamo nezinto ezisetshenziswayo zamabhodlela ngokunengqondo ngokuya ngemisebenzi eyahlukene yokuhlola ukuze kuthuthukiswe ukusebenza kahle kokusebenza futhi bagweme amaphutha noma izingozi ezingadingekile. Ibhodlela elifaniswe ngokunembile lingase libe yingxenye ebalulekile yempumelelo yokuhlolwa.